FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17666783
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGC482314627.30064664291173TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATG182762410.344973388760138TruSeq Adapter, Index 3 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGCCG1436610.8131701170496066TruSeq Adapter, Index 3 (97% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGCC1095940.6203393113505724TruSeq Adapter, Index 3 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT995900.5637132691333787Illumina Single End PCR Primer 1 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACACCTCGTATGC860400.4870156609723457TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACAACTCGTATGC678580.38409935753441926TruSeq Adapter, Index 3 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGCCGTC625570.3540938947402026TruSeq Adapter, Index 3 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACAGCTCGTATGC456310.2582869784498966TruSeq Adapter, Index 3 (97% over 36bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGC338880.19181760482369653TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACACCTCGTATG255130.14441225660608387TruSeq Adapter, Index 3 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTATGCCGTA223080.1262708666314631TruSeq Adapter, Index 3 (96% over 32bp)
ATGATACGGCGACCACCGAGATCATTGATCGGAAGAGCACACGTCTGAACT208010.11774073412233567Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGCGACATCTCGTATGC191150.1081974007378706TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)