FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20428940
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTATG16064527.863609174044273TruSeq Adapter, Index 12 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTATGC3741001.831225702361454TruSeq Adapter, Index 12 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTATGCCGTC701190.34323366753243195TruSeq Adapter, Index 12 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCACCTCGTATG205480.10058280067394589TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1994550.040.0964931
TACGGCG17000.038.9037446
ACGGCGA17650.037.7259337
CGGCGAC18200.036.2150578
TGCCGTC100950.035.7087545
ATGCCGT114700.034.0117244
CGTATGC628100.033.73982245
ATACGGC20100.033.573445
TCTCGTA2301300.033.1901743
ATCTCGT2305700.033.13260742
CATCTCG2306350.033.11384641
GGCGACC19650.033.0845389
GCTCATC2276600.033.07468838
TCGTATG2370700.033.05538645
GCGACCA19550.033.0236410
TCGGAAG2421900.032.9861763
CTCGTAT2368550.032.98394844
ATCGGAA2426050.032.9724432
CGGAAGA2421600.032.9433864
CGTCTGA2402150.032.79598616
Produced by FastQC (version 0.11.5)