FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15786431
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGC8698945.510390537291171TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATG5065323.2086543183826666TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAAACTCGTATGC325010.2058793403018073TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1005850.043.9258345
GATCGGA627200.039.2276151
ACTCGTA44750.033.68305642
AAACTCG46700.032.03561840
AACTCGT49450.030.57252541
TCGTATG1546250.028.52614844
CTCGTAT1527050.028.47792243
CGTCTGA1564050.028.32808315
ACACGTC1568900.028.26919212
ACGTCTG1569700.028.23758314
CACGTCT1572900.028.19443713
TCTCGTA1458700.028.16206742
ATCTCGT1461950.028.10398741
TCACAAC1574700.028.0467629
CACACGT1582300.028.04542711
GTCACAA1577650.027.99431428
GCACACG1585300.027.98525810
CCAGTCA1580150.027.97698625
CAACCAA1573350.027.97001335
AACAACC1576650.027.9528533
Produced by FastQC (version 0.11.5)