FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9234841
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATG137030.1483837133741664TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA18200.034.6120643
CTCGTAT18950.034.42978744
TCGTATG19600.033.74712845
TATCTCG19050.032.59529541
ATCTCGT20200.031.0737542
GATCGGA25500.026.5387521
ACACGTC26400.025.64886513
CGTCTGA26700.025.10790816
ATCGGAA27550.024.5819472
TCGGAAG28600.023.9149513
ACGTCTG28500.023.6010815
CACGTCT29900.022.72172514
CGGAAGA31300.021.9944554
CACACGT33400.020.47541212
GCACACG34900.019.46646311
GTATCTC34300.018.6935440
AAGTATC34350.018.66572438
AGTCACC36950.017.59603528
CAAGTAT38500.017.00467737
CATCAAG40150.016.53007934
Produced by FastQC (version 0.11.5)