FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23912430
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC918210.3839885783251639TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA102150.042.5958141
ACGGCTA102150.041.8441432
ATCGGAA104650.041.5870862
CTCGTAT102300.041.4582442
CGGCTAC103600.041.28822733
CGTATGC103550.041.08804744
TCTCGTA102950.041.0652941
TCGTATG105100.040.50367443
ACACGTC107700.040.11713
TCGGAAG108950.039.990293
ATCTCGT107400.039.55243740
CGTCTGA109900.039.41625616
GTCACGG108550.039.25327729
AGTCACG109800.038.81257228
TCACGGC111550.038.29500230
CGGAAGA113900.038.2523464
ACGTCTG114550.037.73766715
CATCTCG113250.037.5091739
CACGGCT115350.037.1510731
CACGTCT119850.036.143914
Produced by FastQC (version 0.11.5)