FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21625730
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4887722.260141044949697TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA516950.044.434231
ATCGGAA520600.044.1115042
TCGGAAG520900.044.090423
ACACGTC523400.043.8626313
CGTCTGA522900.043.8099116
CTCGTAT518050.043.77445642
CGTATGC520450.043.74180244
TCTCGTA518350.043.73094641
CGGAAGA527150.043.63174
TCGTATG522400.043.59124843
ACGTCTG527250.043.4868715
ATCTCGT521850.043.4377540
CACGTCT529400.043.3145114
GCACACG535400.042.98458511
AATCTCG528150.042.9366539
GTATGCC533700.042.70221345
CACACGT539700.042.6254312
ACTGACC554250.041.23447432
ACCAATC550950.041.22912236
GACCAAT557100.040.9754435
Produced by FastQC (version 0.11.5)