FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20858169
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC508280.24368390149681882TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA56200.041.413351
CTCGTAT57800.040.33558342
CGTATGC58450.040.04102744
TCTCGTA59650.039.16005341
ATCGGAA60500.038.7107852
TCGTATG61550.038.0974643
ACACGTC62700.037.4947813
TCGGAAG63400.037.0465623
ATCTCGT63350.036.87288340
CGTCTGA64600.036.32234216
CGGAAGA66950.035.1493874
CATCTCG68600.034.2806139
ACGTCTG69150.034.0299715
CACGTCT70850.033.34045414
GTATGCC74300.031.71025345
GCACACG77150.030.70523511
CACACGT78450.030.08185412
CTTAGGC87250.027.05371333
ACTTAGG93650.025.39648432
TTAGGCA100800.023.5732934
Produced by FastQC (version 0.11.5)