FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21886359
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG3724001.7015164559806408TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA389100.044.3588071
ATCGGAA393400.043.848452
TCGGAAG395300.043.6832283
CTCGTAT389050.043.53058644
TCTCGTA389150.043.49567843
GTCACGA393450.043.41991429
TATCTCG389800.043.39916641
TCGTATG392350.043.37090345
ACACGTC397500.043.31693613
CGTCTGA397450.043.27143516
ACGAGTG396850.043.1272932
CGGAAGA400550.043.0994384
ATCTCGT393100.043.08062742
CACGAGT397100.043.0604831
AGTCACG398650.042.8761228
ACGTCTG402350.042.76682715
CACGTCT405300.042.46109414
GCACACG408550.042.21143311
CACACGT412200.041.8431112
CGAGTGG413300.041.43797333
Produced by FastQC (version 0.11.5)