FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34590717
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC754960.21825508849672007TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA87550.040.4686241
CTCGTAT87950.039.5735642
CGTATGC88500.039.45330444
ACGGCTA88700.039.4166132
TCGTATG89250.039.14697343
ATCGGAA91950.038.558652
TCTCGTA90400.038.4762741
CGGCTAC91450.038.37908633
ACACGTC97100.036.60804413
ATCTCGT96200.036.22665440
CGTCTGA99150.035.98729316
TCGGAAG99400.035.8723873
GTCACGG98150.035.7585529
AGTCACG99450.035.29050428
CGGAAGA103700.034.4282954
CATCTCG103100.033.8024239
TCACGGC103800.033.76880630
ACGTCTG108800.032.85743715
CACGTCT111700.032.0849414
GTATGCC113500.031.08002545
Produced by FastQC (version 0.11.5)