FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19856403
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG265590.13375534330160402TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA31550.038.775151
CGTCCCG32000.038.25301435
CTCGTAT33150.037.06435444
ATCGGAA34000.036.7901572
GTCCCGA33400.036.71603836
CCCGATC33850.036.4290138
TCCCGAT33900.036.24206537
TCTCGTA34150.036.0449143
TCGTATG35250.034.9190545
CCGATCT35850.034.5239839
ACCCGTC36750.033.73675532
ACACGTC37200.033.0804513
TCGGAAG38450.032.4737473
CACCCGT38100.032.3640231
CGTCTGA38450.032.29756516
ATCTCGT39250.031.41870142
TCACCCG39800.031.03731330
CGGAAGA40500.030.8300134
ACGTCTG41550.030.15860215
CCCGTCC42250.029.34550533
Produced by FastQC (version 0.11.5)