FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21643352
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2221611.0264629988922234TruSeq Adapter, Index 4 (100% over 51bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT367510.16980271817415343Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT239900.043.1050942
CGTATGC243200.042.9549244
TCGTATG241500.042.7822543
TCTCGTA242950.042.50839641
ATCGGAA261100.042.4246522
CGTCTGA261850.042.1478416
GATCGGA264150.042.0362131
ACACGTC263700.041.9188513
TCGGAAG264750.041.830773
ACGTCTG265950.041.5052815
CACGTCT267300.041.3208114
CGGAAGA269550.041.069154
GTATGCC255500.040.9005345
CACACGT271600.040.774112
GCACACG274300.040.38924411
ATCTCGT256500.040.2891340
AATCTCG265000.039.09021839
GACCAAT273750.038.14479435
ACCAATC277650.038.0951136
ACTGACC276550.037.99447632
Produced by FastQC (version 0.11.5)