FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29280188
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC3583731.2239436440776952TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA400600.044.159711
ATCGGAA403750.043.722982
CTCGTAT391600.043.47886742
TCTCGTA393350.043.22252341
CGTATGC397900.043.20315644
ACACGTC409800.043.16171613
TCGGAAG408950.043.127863
TCGTATG394950.043.11577243
TATCTCG396550.043.06660539
CGTCTGA411300.042.9949816
ATCTCGT398450.042.7313940
CGGAAGA412700.042.7250634
ACGTCTG419000.042.24150515
CACGTCT423200.041.80059414
GTATGCC416250.041.12566845
CACACGT431150.041.113112
GCACACG432950.040.9682211
CACTAGC426400.040.4737831
GCTTATC432600.040.10170736
GTCACTA430700.040.0644829
Produced by FastQC (version 0.11.5)