FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24483503
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC810020.3308431804060064TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT91000.042.0234142
ACGGCTA92350.041.7258432
GATCGGA95750.041.21151
TCTCGTA92600.041.2001341
CGTATGC95500.040.3259144
ATCGGAA98200.040.2713242
CGGCTAC96200.040.07924333
ACACGTC99450.039.7873313
AGTCACG98400.039.64045728
CGTCTGA101000.039.17785316
GTCACGG98750.039.15826829
TCGTATG98150.039.0308643
TCGGAAG101550.038.8981323
ATCTCGT98450.038.88907240
TCACGGC100750.038.35859730
ACGTCTG105100.037.71294815
CACGGCT103250.037.38624631
CACGTCT106400.037.3365114
CATCTCG103550.036.97372439
CGGAAGA107500.036.661324
Produced by FastQC (version 0.11.5)