FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9892664
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT207870.21012540201506896Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG25150.042.7726446
ATACGGC26550.040.601985
CACGACG25350.039.67288640
GATACGG27250.039.641584
ACGACGC25850.039.5147841
TGATACG27400.039.4245643
TACACGA26550.038.3034738
ACGGCGA28400.038.106567
GCGACCA27650.038.08157710
CGACGCT26450.037.85284842
CTACACG26950.037.81844337
ACACGAC27100.037.19400839
CGACCAC28750.036.54628411
CGGCGAC30500.035.1877568
GGCGACC30250.034.9572039
ACGCTCT30150.033.8791744
ACCGAGA32450.033.41924316
GACGCTC31400.032.6021343
GATCGGA12300.031.6629311
ACCACCG33950.031.14743613
Produced by FastQC (version 0.11.5)