FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30241638
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36350.034.78740342
GATCGGA37000.034.670851
TCTCGTA37600.033.57100741
CGATCAG37800.033.51154333
GTCACGA38750.032.98029329
CGTATGC39050.032.38212644
ATCGGAA39700.032.308452
TCGGAAG42000.030.6417713
TCGTATG41500.030.41619143
ACGATCA42750.029.89441532
ACACGTC43550.029.3962513
AGTCACG43900.029.2645328
ATCTCGT43500.029.1727340
CGTCTGA45450.028.16871516
TCACGAT46050.027.75214830
CGGAAGA51650.025.1346364
ACGTCTG53100.024.44832415
CACGTCT55200.023.72202514
GTATGCC60000.021.63787845
CACGATC59750.021.57717531
Produced by FastQC (version 0.11.5)