FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15957477
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2921941.8310789355986539TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA338250.044.4281651
ATCGGAA341000.044.0628662
CTCGTAT310000.043.7142542
TATCTCG311200.043.56371739
TCGGAAG345100.043.529033
TCTCGTA311250.043.5277741
CGTATGC312350.043.47190544
TCGTATG312600.043.38669243
ACACGTC346650.043.35189413
CGTCTGA347600.043.20753516
CGGAAGA347900.043.1980934
ATCTCGT314300.043.06236340
ACGTCTG351350.042.75277715
GTATGCC319100.042.62434445
CACGTCT353850.042.45707314
CACACGT355150.042.33965712
GCACACG355200.042.333711
CACTAGC326600.041.70514331
GCTTATC331400.041.3303836
GTCACTA330800.041.21642729
Produced by FastQC (version 0.11.5)