FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27535591
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19800.030.000944
GATCGGA20650.029.0975881
TCGTATG23250.025.83948545
TCTCGTA23500.025.56459443
ATCGGAA24350.024.4885432
TCGGAAG27500.022.008433
CGTCTGA28000.021.69656616
ACACGTC28200.021.62157413
ATCTCGT29750.020.34514842
CGGAAGA31750.019.1332864
ACGTCTG35700.017.64643515
CACGTCT40400.015.81627314
CACACGT49900.012.80501812
GCACACG51100.012.32819711
AATCTCG52700.011.74131841
ACATGTC79200.08.40903832
TCTGAAC86500.07.881710518
AGAGCAC84400.07.7040978
GTCACAT87650.07.59834129
GTCTGAA88700.07.407184617
Produced by FastQC (version 0.11.5)