FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25739677
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG1266390.4919991808755021TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA140350.043.2390671
CTCGTAT139900.042.3041144
ATCGGAA144150.042.0369072
TCTCGTA140750.041.6810543
TATCTCG142300.041.33769641
TCGTATG143450.041.23122445
CGTCTGA147150.041.21466416
TCGGAAG146800.041.1853183
ACACGTC147750.041.00042313
ATCTCGT144100.040.7588942
CGGAAGA151100.040.1027574
ACGTCTG153550.039.56907715
CACGTCT156700.038.7878614
GCACACG157650.038.48315411
CACACGT163350.037.13994612
GTCACAC182800.033.0159629
AGAGCAC194050.031.3803868
AGCACAC200200.030.51752910
GAGCACA202100.030.1527129
CAGTCAC201900.030.00402327
Produced by FastQC (version 0.11.5)