FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31410734
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC599470.19084877163328945TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66050.040.02765342
GTCACGA67400.039.2579229
TCTCGTA67450.038.9310841
GATCGGA69650.038.7805751
CGTATGC69100.038.32508544
CGATCAG69600.037.89047233
AGTCACG72650.036.5750128
ACACGTC74650.036.2263113
ATCTCGT72850.036.07637440
TCGTATG73950.035.90287843
ATCGGAA75450.035.8485372
ACGATCA74550.035.52512732
TCACGAT75050.035.1663430
TCGGAAG78400.034.5523153
ACGTCTG82250.033.01572415
CACGTCT83500.032.71008314
CGTCTGA84500.032.40347716
CACGATC84700.031.40021131
CGGAAGA88250.030.8232424
GTATGCC87200.030.75703245
Produced by FastQC (version 0.11.5)