FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22864211
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8850.021.6224561
TACGGCG1603.8198777E-1119.6918436
CGTATGC11050.017.71418844
TCTCGTA11650.016.99495941
CTCGTAT11350.016.84947642
ATCGGAA12650.015.4777212
TCGTATG13750.014.56299443
ATCTCGT13900.014.24397640
ACACGTC15550.013.3113113
CGTCTGA15300.013.2347116
TCGGAAG15750.013.0028673
CATCTCG18100.011.18735139
CGGAAGA19300.010.7277544
ACGTCTG20650.010.45958715
ACGACGC3258.440262E-68.99960341
ACGGCGA3301.0235641E-58.863447
CACGTCT23300.08.78716614
CGACGCT3502.1488562E-58.35677342
GCACACG25950.08.2366311
CGGCGAC3806.0042436E-57.6971978
Produced by FastQC (version 0.11.5)