FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30561780
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16650.023.7933541
CTCGTAT17100.023.4225442
CGCCAAT17750.023.32886133
TCTCGTA17500.023.01616141
GTCACGC18900.020.95292329
ACGCCAA19800.020.79983932
TATCTCG20750.019.95418439
CGTATGC20850.019.64060444
ATCGGAA20800.019.4697782
TCGGAAG22800.018.0571373
AGTCACG22500.017.89803328
TCGTATG23050.017.864343
CGTCTGA23300.017.86380616
ATCTCGT23200.017.8463440
ACACGTC22950.017.84225713
ACGTCTG28800.014.92106415
CACGTCT33050.012.52585714
GTATGCC40450.011.12489845
CGGAAGA38100.011.1010514
ACGACGC2057.7909604E-49.87885441
Produced by FastQC (version 0.11.5)