FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18554756
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG5550.036.900436
GATCGGA21100.035.5289961
CTCGTAT21950.034.0303242
CGTATGC21900.034.0052844
ATCGGAA22550.033.3336562
TCTCGTA22800.032.95900341
ACACGTC23950.031.75218813
TATCTCG23600.031.65108139
TCGTATG23650.031.58416643
ACGACGC6600.029.31682841
TCGGAAG26400.029.239723
ATCTCGT26150.028.90879840
CGTCTGA27400.027.75419216
CACGACG7000.027.32016640
CGGAAGA27850.027.313324
CGACGCT7250.026.68842342
CGGCGAC8100.025.8332548
ACGTCTG30700.025.21056215
ACGGCGA8250.025.0910367
ATACGGC9100.022.7525185
Produced by FastQC (version 0.11.5)