FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14946645
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC991760.6635335220713411TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA110350.042.9509621
CTCGTAT109550.042.57215542
ATCGGAA111250.042.4871372
TCTCGTA109800.042.41375441
CGTATGC110550.042.12600744
TCGGAAG113250.041.7765433
TCGTATG111850.041.656543
ACACGTC114050.041.44418713
ATCTCGT112800.041.26578540
CGTCTGA114350.041.2577316
CGGAAGA117400.040.2806134
ACGTCTG117950.039.94126515
CACGTCT118900.039.6599814
GTATGCC120950.038.5403245
AATCTCG121350.038.45100439
CACACGT123300.038.42613612
GCACACG124950.037.88269811
ACCAATC131650.035.54462436
GACCAAT132500.035.4537435
ACTGACC134000.035.07365832
Produced by FastQC (version 0.11.5)