FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16250474
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA17700.033.330091
TCTCGTA18100.031.57159241
CTCGTAT18050.031.53430642
CGTATGC18150.031.36056744
ACACGTC19500.030.11169613
ATCGGAA19650.030.1107222
TACGGCG3450.028.039876
TCGGAAG21200.027.6969933
ATCTCGT20950.027.49142540
TCACGAT21950.026.44428635
TCGTATG21700.026.43750443
CGTCTGA23450.024.94463716
ACGTCTG23900.024.75737215
ACGATCT25250.023.43291737
CGGAAGA25700.023.1099384
CACGTCT25850.022.97682614
CACGATC26050.022.79943836
ACGACGC4250.021.70382341
GTATGCC27900.020.72313945
CACGACG4400.020.45260640
Produced by FastQC (version 0.11.5)