FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20960856
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC351760.1678175738624415TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA44300.041.288821
CTCGTAT43800.039.8516342
TCTCGTA44900.038.82839241
CGTATGC45300.038.43278544
ATCGGAA48200.038.1344572
TCGTATG46550.037.5942943
ATCTCGT47350.036.7717540
ACACGTC50250.036.51843313
TCGGAAG51500.035.7315033
CGTCTGA52050.035.29875616
CGGAAGA52850.034.776214
ACGTCTG54600.033.81493815
CACGTCT58050.031.72778514
TACGGCG2600.029.4178416
GCACACG62900.029.28136811
CACACGT63500.029.18176712
AATCTCG60400.029.12519839
GTATGCC63050.027.89566445
AGTCACC85300.021.06472628
AGAGCAC90150.020.7546628
Produced by FastQC (version 0.11.5)