FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20375441
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC1151.5993464E-719.56436541
GATCGGA6250.015.1283251
TACGGCG1656.907505E-613.63931756
CGACGCT1852.2482862E-512.16163342
CTCGTAT7850.012.03769344
CACGACG2201.0622574E-511.24951140
TCTCGTA8600.010.98789443
ACGGCGA2056.4109525E-510.975517
ATCGGAA8900.010.6202772
TCGTATG9850.010.27874145
ACACGTC11250.09.99956513
CGTCTGA10600.09.97598116
TATCTCG9300.09.67699841
GTCACGT10600.09.55147129
ATCTCGT10450.09.47327142
TCGGAAG10750.09.2112973
CGGCGAC2453.8742527E-49.1835448
AGTCACG10300.08.95592128
ACGTCTG14400.07.968403315
CACGTCT15800.07.68953914
Produced by FastQC (version 0.11.5)