FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24123115
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC387980.16083329205204222TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA49800.037.7844961
CGTATGC49600.037.1523944
CTCGTAT49400.036.98681342
TCTCGTA50050.036.55142241
TCGTATG50050.036.46120543
ATCGGAA54100.034.8491062
ACACGTC54700.034.26304613
TCGGAAG56900.033.0936473
CGTCTGA56650.033.0835116
ATCTCGT55350.033.01095240
CATCTCG59700.030.75748339
CGGAAGA62200.030.3097484
ACGTCTG62900.029.97504815
CACGTCT65100.029.0313114
GTATGCC66800.027.78772545
GCACACG69150.027.39373811
CACACGT70300.026.88256312
GATCATC87400.021.3972936
TACGGCG5350.020.1844146
CCAGATC103650.018.36858233
Produced by FastQC (version 0.11.5)