FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31037416
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37400.033.2807041
CTCGTAT35950.032.48290342
CGTATGC36000.032.43716444
TCTCGTA36900.031.58671641
ATCGGAA40050.031.4630832
TCGTATG37550.031.15823243
TATCTCG38750.030.07865739
ACACGTC47150.026.67393713
ATCTCGT44950.025.98018540
CGTCTGA49500.025.22611416
ACGTCTG54000.023.2069715
TCGGAAG55100.023.1522963
CGGAAGA57550.022.049384
GTATGCC56800.021.15294645
CACGTCT60650.020.73662214
CACACGT63250.020.23962412
CACTAGC66350.018.00746531
GTCACTA69150.017.47278829
GCACACG74100.017.33678611
ACTAGCT73350.016.35049832
Produced by FastQC (version 0.11.5)