FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21957733
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC3066721.3966469125023062TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA415650.044.337171
ATCGGAA418500.044.0259672
ACGGCTA323150.043.38677632
ACACGTC422150.043.37147513
TCTCGTA323800.043.31993541
CTCGTAT323700.043.2208242
CGTCTGA423550.043.19673516
TCGTATG323800.043.13759243
TCGGAAG427700.043.0678063
CGGAAGA428300.042.9969644
CGTATGC326650.042.97474744
ATCTCGT328250.042.74695240
ACGTCTG429600.042.58268415
AGTCACG339350.042.51396628
CGGCTAC336000.042.511333
GTCACGG335500.042.06982429
CACGTCT436900.041.9123814
CACACGT438700.041.83215312
GTATGCC342500.041.71518345
GCACACG442950.041.4714211
Produced by FastQC (version 0.11.5)