FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24075196
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1138520.47290165363555087TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA124700.042.1588751
ATCGGAA125400.041.990012
CTCGTAT123950.041.80542842
CGTATGC124150.041.7199544
TCTCGTA124850.041.46793441
TCGTATG125250.041.35355443
TATCTCG126000.041.14268539
ACACGTC131150.040.0238713
ATCTCGT129950.039.8574740
CGTCTGA133300.039.48164416
TCGGAAG134500.039.26113
CGGAAGA138300.038.0195854
GTATGCC138050.037.66593645
ACGTCTG140450.037.43773715
CACGTCT141600.037.22902314
CACACGT145150.036.22488812
GTCACTA148300.035.21343229
CACTAGC150350.034.8380631
GCACACG151250.034.77879311
GCTTATC152700.034.11001236
Produced by FastQC (version 0.11.5)