FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21615015
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG4000.035.4447486
GATCGGA11150.030.890691
ACGACGC5200.027.25850341
CGACGCT5150.027.08627542
CTCGTAT13500.025.332342
CACGACG5850.024.22978440
ACGGCGA5900.024.0257
CGTATGC14250.023.99907944
ATCGGAA14400.023.7548582
TCTCGTA14800.023.10716841
TCGTATG15500.021.91846343
CGGCGAC6600.021.8175968
ACACGTC17800.019.97109613
TCGGAAG17750.019.7786923
ATCTCGT17750.019.39357640
ATACGGC8350.018.0575855
CGGAAGA19550.017.8425244
CGTCTGA19700.017.81653416
GCGACCA8750.016.97073710
GGCGACC9000.016.749329
Produced by FastQC (version 0.11.5)