FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23303493
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC703800.30201480953949694TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA83150.041.9924741
CGATCAG81900.041.48872833
GTCACGA82150.041.2555829
CTCGTAT82400.041.1119642
CGTATGC83500.040.84037844
TCTCGTA83300.040.67100541
ATCGGAA87300.040.0734942
AGTCACG85900.039.60993628
ACACGTC88650.039.54811513
TCGTATG85950.039.49278343
ATCTCGT86300.039.25718340
TCGGAAG89350.039.200983
ACGATCA87300.038.99204332
TCACGAT87850.038.63014630
ACGTCTG91900.038.0271615
CGTCTGA92850.037.68652316
CACGTCT94050.037.3252314
CACGATC92600.036.84287331
CGGAAGA97700.035.873664
GTATGCC98800.034.44255445
Produced by FastQC (version 0.11.5)