FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840013
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47160.5614198827875283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39150.46606421567285267No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCATA33390.3974938483094904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28130.3348757697797534No Hit
CATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAG27770.3305901218195433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27320.3252330618692806No Hit
GTGCACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26790.3189236357056379No Hit
ATCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCAC24820.2954716177011546No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23810.2834479942572318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23550.28035280406374663No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22990.2736862405700864No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC22800.27142437081330884No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG22520.26809108906647877No Hit
CTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCC22190.2641625784362861No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21600.2571388776126084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21450.2553531909625208No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA20850.2482104443621706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20630.2455914372753755No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20520.24428193373197796No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20440.24332956751859794No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19670.23416304271481514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19360.2304726236379675No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18550.2208299157274947No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA17920.21333003179712695No Hit
GTCTGGGACCCCAGAGTCCCGGATAGAAGCCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA17340.20642537675012174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17070.20321114077996413No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG16660.19833026393639144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16580.19737789772301143No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGA16000.1904732426760062No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15950.18987801379264369No Hit
GCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGT15630.18606854893912356No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTG15190.18083053476553337No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC15140.18023530588217088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15100.17975912277548087No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCATATA15080.17952103122213586No Hit
GTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC14940.17785439034872078No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14910.17749725301870328No Hit
AGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC14890.17725916146535828No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC14600.17380683394185567No Hit
GTATATGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA14510.17273542195180314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14380.1711878268550606No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13840.16475935491474536No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13400.1595213407411552No Hit
GTCCCGGATAGAAGCCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCC13340.1588070660811202No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGCTTCTATCCGGGAC13210.15725947098437762No Hit
GTTTATTACTGCATGCAAGGTGCACACTGGCCTCCGTACACTTTTGGCCA13000.15475950967425506No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12590.14987863283068237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12580.14975958705400988No Hit
CTGTATATGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCC12570.1496405412773374No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTCCACT12530.14916435817064735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12520.14904531239397487No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12010.14297397778367715No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11990.14273588623033215No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11540.13737882628006948No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11480.13666455162003446No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGCTT11400.13571218540665442No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11400.13571218540665442No Hit
GGTCTAATCAAAGCCTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTC11290.13440268186325688No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11100.1321408121064793No Hit
TTATTACTGCATGCAAGGTGCACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA11070.1317836747764618No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11060.13166462899978928No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10710.12749802681625164No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10590.1260694774961816No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10520.12523615705947408No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10400.12380760773940402No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG10200.12142669220595395No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10180.12118860065260895No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10150.12083146332259144No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10080.1199981428858839No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10000.11904577667250388No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9970.11868863934248637No Hit
GCCTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGG9960.11856959356581387No Hit
AATTTATAGGGCTTCTATCCGGGACTCTGGGGTCCCAGACAGATTCAGCG9910.11797436468245134No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9870.11749818157576132No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG9830.11702199846907133No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9760.1161886780323638No Hit
GATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTGCACACTGG9730.11583154070234628No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9700.11547440337232877No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9690.11535535759565625No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9620.11452203715894872No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9570.11392680827558622No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9530.1134506251688962No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9450.11249825895551617No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9420.11214112162549865No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9410.11202207584882615No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9400.11190303007215364No Hit
GTATTATTGTGCGAGAGACGTAGTAGTAGTAGCCGCTTCGGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGT9170.10916497720868605No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8970.10678406167523596No Hit
GGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGCT8940.10642692434521847No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTC8900.10595074123852845No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8790.10464123769513092No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8770.10440314614178589No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8760.10428410036511339No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8700.10356982570507837No Hit
GGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTC8690.10345077992840587No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAG8620.10261745949169834No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8600.10237936793835334No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8580.10214127638500832No Hit
CAATAATACATGGCCGTGTCGTCAGATGTCAGGCTCCTCAACTCCATGTA8530.10154604750164582No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8530.10154604750164582No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8530.10154604750164582No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8480.1009508186182833No Hit
AGGTGCACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8470.1008317728416108No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8440.10047463551159327No Hit
CCATTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTC8430.10035558973492077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)