FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840013
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45730.5443963367233603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29200.34761366788371134No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCATA28710.34178042482675863No Hit
GTGCACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT27230.3241616498792281No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27230.3241616498792281No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26350.3136856215320477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24870.29606684658451715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23540.28023375828707414No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG23410.27868616319033157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23240.276662384986899No Hit
ATCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCAC22340.26594826508637365No Hit
CATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAG22030.26225784600952606No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21080.25094849722563817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20400.2428533844119079No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA20390.24273433863523539No Hit
CTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCC20020.23832964489835273No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19420.23118689829800254No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19330.23011548630794998No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18810.2239251059209798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18350.21844900019404465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18330.21821090864069959No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18070.2151157184472145No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17410.20725869718682924No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17240.20523491898339669No Hit
GTCTGGGACCCCAGAGTCCCGGATAGAAGCCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA16780.1997588132564615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16720.19904453859642648No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG16330.19440175330619883No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16190.1927351124327838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16090.19154465466605874No Hit
GTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC16070.19130656311271374No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA16010.1905922884526787No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15890.18916373913260867No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15330.18249717563894843No Hit
AGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC15210.1810686263188784No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14900.1773782072420308No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCATATA14660.17452110860189068No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTG14660.17452110860189068No Hit
GTATATGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA14270.16987832331166303No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13930.1658307669047979No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC13790.16416412603138286No Hit
GCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGT13240.15761660831439514No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGA13160.1566642421010151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13120.15618805899432509No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13070.1555928301109626No Hit
GTTTATTACTGCATGCAAGGTGCACACTGGCCTCCGTACACTTTTGGCCA13010.15487855545092755No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC12970.15440237234423754No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12370.1472596257438873No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA12340.14690248841386977No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12060.14356920666703968No Hit
GTCCCGGATAGAAGCCCTATAAATTAGGCGCCTTGGAGATTGGCCTGGCC11650.13868832982346702No Hit
GGTCTAATCAAAGCCTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTC11490.13678359739670695No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11470.13654550584336195No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11350.1351169565232919No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGCTTCTATCCGGGAC11130.13249794943649681No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTCCACT11080.1319027205531343No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGGCTT10960.13047417123306426No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10960.13047417123306426No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10930.13011703390304674No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10860.1292837134663392No Hit
CTGTATATGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCC10850.1291646676896667No Hit
TTATTACTGCATGCAAGGTGCACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA10810.1286884845829767No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10430.12416474506942154No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10180.12118860065260895No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10160.12095050909926394No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTGCACAC10140.12071241754591892No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10130.12059337176924642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10120.12047432599257392No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10010.11916482244917637No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10000.11904577667250388No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9980.11880768511915887No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9870.11749818157576132No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9850.11726009002241632No Hit
AATTTATAGGGCTTCTATCCGGGACTCTGGGGTCCCAGACAGATTCAGCG9810.1167839069157263No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9740.11595058647901878No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG9670.11511726604231125No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9570.11392680827558622No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9570.11392680827558622No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9440.11237921317884367No Hit
GCCTCATATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGG9250.1101173434220661No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8970.10678406167523596No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8900.10595074123852845No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG8880.10571264968518344No Hit
GATTTCACACTGGAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTGCACACTGG8870.10559360390851094No Hit
GTATTATTGTGCGAGAGACGTAGTAGTAGTAGCCGCTTCGGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGT8790.10464123769513092No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8770.10440314614178589No Hit
GCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCC8690.10345077992840587No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCATTGGGGATGTTGTGATGACTCAGTCTC8670.10321268837506087No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAG8630.10273650526837085No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8570.10202223060833582No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8490.1010698643949558No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8440.10047463551159327No Hit
GGCCAGTGTGCACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTC8420.10023654395824827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG200.007737475451.0965237
GGTATCA5950.047.549481
CTATACC603.214318E-739.7417374
GTATCAA15600.038.4567641
CGAAACC708.638295E-636.7894569
TATACCT656.0191087E-736.684685
TGAATTA701.0693839E-634.08667821
CCTATAC701.0748772E-634.0643463
CGGCAAA952.0563901E-433.91428870
ATACCTT652.5313657E-531.4440126
TATCAAC18850.031.2744752
TGGTATC1650.030.9786572
CCGAAAC752.93096E-530.77589668
AATCGTT450.00461111630.68138939
GACTGCG450.004918385330.2794177
TACAATC450.004918385330.2794174
TCTCGCG3450.029.8838970
TCAACGC19950.029.0272624
ATCAACG20000.028.9546933
AATGGTA3800.028.82714370
Produced by FastQC (version 0.11.5)