FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913452
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC117030.6116171192169964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC74220.3878853506646626No Hit
GCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGC74040.38694464245771515No Hit
ACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCACTTCTGGTGTCCCACACCTGACAGAAGTAGTCAGC72970.3813526547830831No Hit
GTTCTGGAGAGGGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAG68060.355692225360239No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCACCTGTTCTGG66080.34534443508381707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64590.33755746159297434No Hit
GGATTTGTGGCCTCCTATGAGCTGATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCAC61150.3195794825268677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58350.3049462437521297No Hit
CCTATGAGCTGATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACA58260.3044758896486559No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTC55530.29020848184328635No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48190.25184849162665174No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT47530.24839922820117777No Hit
GGATGAGGCTGACTACTTCTGTCAGGTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGA44820.2342363435299135No Hit
CCCCAGGACAGACAGCCAGTGTCACCTGTTCTGGAGAGGGATTGGGGGAA43870.22927149465991306No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42600.22263427564422836No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42220.22064833609622816No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT41630.21756490364012265No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG41020.2143769480499119No Hit
GACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCACTTCTGGTGTCCCACACCTGACAGAAGTAGTCAG40840.21343623984296448No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT39080.20423820404170054No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37700.19702610778843682No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37470.1958240917462262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC37230.19456981413696295No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG37150.19415172160054184No Hit
CTCCAGAACAGGTGACACTGGCTGTCTGTCCTGGGGACACGGACACTGAGGGTGTCTGAATCAGCTCATAGGAGG36640.19148638168085744No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTCTGGATGAGGCTGACTAC36350.189970796236331No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36190.1891346111634888No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36140.18887330332822563No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA36070.1885074723588572No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGG36060.18845521079180455No Hit
CTTCTGGTGTCCCACACCTGACAGAAGTAGTCAGCCTCATCCAGAGCCTGGGTCCCGCTGATGGTCAGAGTGG35750.18683510221317284No Hit
GCCTCATCCAGAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC34150.17847325148475113No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG33750.17638278880264568No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33390.1745013723887508No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA33230.17366518731590863No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG32480.16974556978696093No Hit
CACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA32460.16964104665285568No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGGCCTCCTATGAGCTGATT32180.16817772277538187No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31650.16540785972159217No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30760.1607565802539076No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30730.16059979555274967No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA30110.15735957839548628No Hit
GCAGAAGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGACTTCATGCGGCCCTCAGGGATCCCTGAGCGATT29620.15479876160990713No Hit
GATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCA29440.15385805340295966No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCA29180.15249925265959113No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27570.1440851403641168No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27460.1435102631265378No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT26590.1389635067929585No Hit
GTCCCACACCTGACAGAAGTAGTCAGCCTCATCCAGAGCCTGGGTCCCGC25940.13556650493453715No Hit
GTGTCACCTGTTCTGGAGAGGGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAGCCTGGCCAGCCCCCG25630.13394639635590544No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGGCCTCCTATGAG25410.13279664188074747No Hit
AGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGACTTCATGCGGCCC25380.13263985717958957No Hit
GACTACTTCTGTCAGGTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAG25320.13232628777727376No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG25270.13206497994201055No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25200.13169914897264212No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23870.12474836055464154No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT23720.12396443704885203No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23690.12380765234769411No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23280.12166492809853605No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTC23240.12145588183032552No Hit
GGCTTCTGCTGATACCAGGAAACTAACTTTTCCCCCAATCCCTCTCCAGAACAGGTGACACTGGCTGTCTGTC22970.12004481951990434No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22950.11994029638579906No Hit
GCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTC22690.11858149564243053No Hit
CCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGGCTGGCC22430.11722269489906202No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC22150.11575937102158822No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21790.11387795460769332No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG21590.11283272326664061No Hit
CCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGG21540.11257141543137743No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21420.11194427662674579No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT21070.11011512177990354No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21060.11006286021285092No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC20990.10969702924348247No Hit
GTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAGCCCAAGGCTGCCCC20920.109331198274114No Hit
GGCCAGGCTTCTGCTGATACCAGGAAACTAACTTTTCCCCCAATCCCTCTCCAGAACAGGTGACACTGGCTGT20860.10901762887179821No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG20630.10781561282958757No Hit
GAAGTAGTCAGCCTCATCCAGAGCCTGGGTCCCGCTGATGGTCAGAGTGG20630.10781561282958757No Hit
GGGATCCCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGG20610.10771108969548229No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20390.10656133522032431No Hit
GTCCTGGGGACACGGACACTGAGGGTGTCTGAATCAGCTCATAGGAGGCCACAAATCCTGTGCAGTAAGCAAGGA20380.10650907365327168No Hit
GCACAGGATTTGTGGCCTCCTATGAGCTGATTCAGACACCCTCAGTGTCC20180.10546384231221897No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCT20130.10520253447695578No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19990.10447087253821889No Hit
CACTGAGGGTGTCTGAATCAGCTCATAGGAGGCCACAAATCCTGTGCAGT19600.10243267142316609No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCACCTGTTCTGGAGAGGG19600.10243267142316609No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT19590.10238040985611346No Hit
GGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGA19580.10232814828906081No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC19350.1011261322468502No Hit
TCCCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGGCTGGCCAGGCTTCTGCTGATACCAGGAAA19300.100864824411587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4750.050.28380670
TCGTATG4350.044.2738740
CGTATGC4450.044.16010341
GCCGTCT4750.041.82145746
ATGCCGT4750.040.91751544
TCTCGTA4450.039.9956838
ACGAGAC4850.039.62532822
CGAGACG4800.039.3397823
CTTATAC5100.039.0122531
GTATCAA48600.038.857151
ATCTCCG5000.038.4305410
CCGTCTT5100.038.372747
CCCACGA4950.038.13869519
GGAGCTA5150.037.42170329
CACGAGA5300.036.2571221
CTACATC5000.035.99230233
GACGGAG5300.035.65177526
ATCTCGT5000.035.5141837
GTATGCC5500.035.1882842
TATCAAC53550.035.1292652
Produced by FastQC (version 0.11.5)