FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913452
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC108970.5694942961725719No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC74600.38987129021266276No Hit
GTTCTGGAGAGGGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAG70770.3698551100315033No Hit
GCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGC69550.3634791988510817No Hit
ACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCACTTCTGGTGTCCCACACCTGACAGAAGTAGTCAGC66170.34581478918729086No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG65580.3427313567311853No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCACCTGTTCTGG61510.3214608989407626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61130.3194749593927624No Hit
GGATTTGTGGCCTCCTATGAGCTGATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCAC60730.31738449671065694No Hit
CCTATGAGCTGATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACA53630.2802787841032856No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTC50370.2632415132441263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46400.24249367112422993No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG46250.24170974761844038No Hit
GGATGAGGCTGACTACTTCTGTCAGGTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGA43750.22864435585528148No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43330.22644937003907076No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43060.22503830772864958No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42510.2221639215407546No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT41540.21709454953664897No Hit
GACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCACTTCTGGTGTCCCACACCTGACAGAAGTAGTCAG39150.204604035011069No Hit
CCCCAGGACAGACAGCCAGTGTCACCTGTTCTGGAGAGGGATTGGGGGAA38600.201729648823174No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37640.196712538386121No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37230.19456981413696295No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36760.19211352048548905No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36490.1907024581750679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36200.18918687273054147No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35830.18725319474959393No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35690.186521532810857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35220.18406523915938314No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT35030.18307226938538307No Hit
CTCCAGAACAGGTGACACTGGCTGTCTGTCCTGGGGACACGGACACTGAGGGTGTCTGAATCAGCTCATAGGAGG34340.1794662212587512No Hit
CTTCTGGTGTCCCACACCTGACAGAAGTAGTCAGCCTCATCCAGAGCCTGGGTCCCGCTGATGGTCAGAGTGG34210.17878682088706693No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG34050.17795063581422477No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33290.17397875671822446No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTCTGGATGAGGCTGACTAC32960.17225412500548745No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGG32910.17199281717022427No Hit
GCCTCATCCAGAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC32050.1674983224036976No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG31790.16613952166032908No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31350.1638400127100131No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31170.16289930450306567No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGGCCTCCTATGAGCTGATT30130.15746410152959156No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30110.15735957839548628No Hit
CACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA29260.15291734519601224No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29220.1527082989278017No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29070.15192437542201215No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC28960.15134949818443316No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28780.15040878997748572No Hit
GTGTCACCTGTTCTGGAGAGGGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAGCCTGGCCAGCCCCCG28650.14972938960580146No Hit
GCAGAAGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGACTTCATGCGGCCCTCAGGGATCCCTGAGCGATT27210.1422037239502219No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27020.14121075417622184No Hit
GATTCAGACACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCA26710.1395906455975901No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25840.1350438892640108No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG25790.1347825814287476No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTCCCTCCGCCGAAAAGCGCA25750.13457353516053708No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25020.13075844076569468No Hit
GGCTTCTGCTGATACCAGGAAACTAACTTTTCCCCCAATCCCTCTCCAGAACAGGTGACACTGGCTGTCTGTC24840.12981773255874723No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24390.1274659620413786No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATTTGTGGCCTCCTATGAG24380.12741370047432599No Hit
GTCCCACACCTGACAGAAGTAGTCAGCCTCATCCAGAGCCTGGGTCCCGC24000.12542776092632582No Hit
AGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGACTTCATGCGGCCC24000.12542776092632582No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23240.12145588183032552No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAGTCAGCTTGGTC23180.1211423124280097No Hit
GACTACTTCTGTCAGGTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAG23070.1205674351904307No Hit
GTGTGGGACACCAGAAGTGCGCTTTTCGGCGGAGGGACCAAGCTGACTGTCCTAGGTCAGCCCAAGGCTGCCCC22840.11936541914822008No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22080.11539354005221977No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21590.11283272326664061No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21570.11272820013253533No Hit
GGGATCCCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGG21440.11204879976085107No Hit
GCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTC21360.11163070722443No Hit
GTCCTGGGGACACGGACACTGAGGGTGTCTGAATCAGCTCATAGGAGGCCACAAATCCTGTGCAGTAAGCAAGGA21260.11110809155390362No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT21160.11058547588337726No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20730.10833822850011393No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC20640.10786787439664022No Hit
GCACAGGATTTGTGGCCTCCTATGAGCTGATTCAGACACCCTCAGTGTCC20370.10645681208621904No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20300.10609098111685059No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGTGTCACCTGTTCTGGAGAGGG19960.10431408783706096No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCT19930.10415730313590307No Hit
GGCCAGGCTTCTGCTGATACCAGGAAACTAACTTTTCCCCCAATCCCTCTCCAGAACAGGTGACACTGGCTGT19910.1040527800017978No Hit
CCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGG19900.10400051843474517No Hit
CCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGGCTGGCC19840.10368694903242935No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG19780.10337337963011353No Hit
GGATTGGGGGAAAAGTTAGTTTCCTGGTATCAGCAGAAGCCTGGCCAGCCCCCGCTGTTGGTCATCTATCGAGA19740.10316433336190298No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19710.10300754866074509No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19590.10238040985611346No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19590.10238040985611346No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19430.10154422478327127No Hit
TCCCTGAGGGCCGCATGAAGTCTCGATAGATGACCAACAGCGGGGGCTGGCCAGGCTTCTGCTGATACCAGGAAA19250.10060351657632384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)