FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315382
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCGACATCGGGGCAGGTTATGATGTACAGTGGTATCAGCAGATTCCAGGAACAGC48860.37145103095526627No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41960.3189947863054231No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAACTTGGTCCCTC40830.3104041259497241No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40230.3058427133714769No Hit
GTCATAAGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG36750.27938652041764295No Hit
GTAATAGACGGCCGTGTCAGCGGCAGTCACAGACTTCAGTTGCAGGGAGA33850.25733969295611464No Hit
GCCTTGGGCTGACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCACACTCAGGCTCCTGTCATAAGACTGG33070.25140985660439324No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32780.24920517385824043No Hit
GTCTTATGACAGGAGCCTGAGTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCTTACGTCAGCCCAAGGCTGC32770.2491291503152696No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32720.2487490326004157No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32300.2455560437956426No Hit
GACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCACACTCAGGCTCCTGTCATAAGACTGGCAGTAATAAT30510.23194782960387172No Hit
GATTATTACTGCCAGTCTTATGACAGGAGCCTGAGTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCTTACGT30250.22997121748663127No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30230.2298191704006897No Hit
GGTTATGATGTACAGTGGTATCAGCAGATTCCAGGAACAGCCCCCAAAAACGTCATCTATGGAAACAGTAATCGG29360.2232051221622312No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29340.22305307507628963No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26770.20351502453279732No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTA26020.19781325880998826No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25970.19743314109513435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25900.19690097629433884No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25850.19652085857948487No Hit
ATGATGTACAGTGGTATCAGCAGATTCCAGGAACAGCCCCCAAAAACGTCATCTATGGAAACAGTAATCGGCCCT25620.19477231709115678No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25120.19097113994261744No Hit
GTCTATTACTGCGCGCGACAGAGGTGGGGTGTCAGAGGGATTGCAGTGGTGGCAGAAGCCCTTGACCTCTGGGGC24990.1899828338839972No Hit
GTCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24670.18755008050893202No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG24620.18716996279407808No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24430.18572551547763314No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23920.18184831478612296No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23360.1775909963797589No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23240.17667871386410944No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23210.17645064323519707No Hit
CAGTAATAGACGGCCGTGTCAGCGGCAGTCACAGACTTCAGTTGCAGGGA23060.17531029009063526No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23000.17485414883281056No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22950.17447403111795662No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22660.17226934837180377No Hit
GTACAGTGGTATCAGCAGATTCCAGGAACAGCCCCCAAAAACGTCATCTA22570.1715851364850667No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG22450.17067285396941723No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGT21880.16633951202008238No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21120.16056172275430255No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21090.1603336521253902No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20910.158965228351916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20900.15888920480894522No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20540.1561523572619969No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCGACATCGGGGCAGGTTATGATGTACAGTGGTATCAG20450.1554681453752598No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20140.15311141554316543No Hit
CTATTACTGCGCGCGACAGAGGTGGGGTGTCAGAGGGATTGCAGTGGTGG19750.1501464973673047No Hit
TTCCAGGAACAGCCCCCAAAAACGTCATCTATGGAAACAGTAATCGGCCC19650.14938626193759683No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCACA19610.14908216776571367No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCTTAT19530.1484739794219474No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18910.14376051975775858No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18690.14208800181240128No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18590.1413277663826934No Hit
ACTCAGGACAATCGCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC18580.14125174283972258No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG18140.13790670694900795No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17890.13600611837473828No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17880.1359300948317675No Hit
AGGCAGCACTCAGGACAATCGCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17830.13554997711691358No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17660.13425757688641018No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17610.13387745917155625No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17470.13281312956996522No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17340.131824823511345No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17110.1300762820230169No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17070.12977218785113373No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17070.12977218785113373No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16920.12863183470657194No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16780.1275675051049809No Hit
ATACCACACTCAGGCTCCTGTCATAAGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGG16730.12718738739012697No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCACACTCAGG16690.12688329321824385No Hit
TCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16680.12680726967527306No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16380.12452656338614942No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16320.1240704221283247No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16100.12239790418296739No Hit
GGACAATCGCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT16080.12224585709702582No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16060.12209381001108423No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15180.11540373822965495No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG14780.11236279651082347No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14750.1121347258819111No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14660.11145051399517403No Hit
CTATTATAGTGGGAGCACCAACGACAACCCGTCCCTCCGGCGTCGAGTCACCATTTCCGCAGACATGTCGAAGAA14630.11122244336626166No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14600.1109943727373493No Hit
GTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC14530.1104622079365538No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14520.11038618439358301No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG14480.11008209022169986No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14410.10954992542090433No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG14310.10878968999119647No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14270.10848559581931332No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14160.10764933684663466No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14150.10757331330366388No Hit
CCCCTGAGGGCCGATTACTGTTTCCATAGATGACGTTTTTGGGGGCTGTTCCTGGAATCTGCTGATACCACTGTA14090.10711717204583916No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13960.10612886598721893No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCGCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC13930.10590079535830657No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13830.10514055992859869No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13770.10468441867077397No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13650.10377213615512453No Hit
CCACTGTACATCATAACCTGCCCCGATGTCGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC13530.10285985363947509No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13480.10247973592462113No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13280.10095926506520539No Hit
CAGTAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCA13190.10027505317846831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)