FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121787
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC94580.8431190591440265No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGCCACTACTTGTATATGAGCAA77210.6882768297368396No Hit
GATTACTACTGTTGCTCATATACAAGTAGTGGCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGT50440.44963972661476737No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50220.4476785699959083No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49300.43947736958977063No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG46070.4106840246856132No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATTGTCAGGGAGG42650.38019695361062306No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40770.363437978867646No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC40680.36263568752356734No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40560.3615659657314624No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37010.3299200293816919No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36330.323858272559764No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC34510.30763415871283945No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34400.30665358040340995No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32060.285794005457364No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATTTT31410.2799996790834624No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30640.27313563091745585No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29080.2592292476200919No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27950.24915603407777054No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27780.2476405948722886No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27680.2467491600455345No Hit
CTCCTGCACTGGAACCAGCAGTGATTTTGGGAATTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC27650.24648172959750828No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27280.2431834207385181No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC27150.24202455546373777No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATTTTGGGAATTATAACCTTGTCTCCTGGTACCAACAACA27010.24077654670628204No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26940.24015254232755417No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT26830.23917196401812466No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26800.23890453357009842No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26500.2362302290898361No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25220.22481986330738368No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24780.2208975500696656No Hit
TATACAAGTAGTGGCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC24410.21759924121067548No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23530.20975461473523938No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22790.20315799701725906No Hit
ACTTGTATATGAGCAACAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCC22790.20315799701725906No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC22610.20155341432910168No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22380.19950311422756725No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22180.19772024457405907No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21690.19335221392296398No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGA21080.18791446147976398No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGC20920.18648816575695742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20570.18336814386331807No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG20270.1806938393830558No Hit
GCTTGGTCCCTCCGCCGAAGACCAAAGTGCCACTACTTGTATATGAGCAACAGTAGTAATCAGCCTCGTCCTCAG19680.1754343739052066No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC19630.17498865649182954No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19390.1728492129076197No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19370.1726709259422689No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC18950.16892689966990168No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18940.16883775618722627No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18810.16767889091244595No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGCCACTACTTGTATATGAGCAACAGTAGTAAT18770.1673223169817443No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGCCA18680.1665200256376656No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT18600.16580687777626232No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18450.16446972553613118No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18420.16420229508810497No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17990.16036912533306233No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17950.1600125514023607No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAATTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA17800.15867539916222956No Hit
GCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCA16820.14993933786003938No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGATCCAGACACGGAGGCAGG16790.14967190741201317No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16490.14699760293175085No Hit
GGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16280.14512558979556725No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16070.14325357665938362No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15680.13977698083504267No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15580.13888554600828856No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15530.1384398285949115No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15480.13799411118153448No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15380.13710267635478035No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15360.13692438938942955No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTTTAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTCTTGA15340.13674610242407872No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC15050.13416094142649182No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14940.13318036311706233No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14930.13309121963438691No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC14860.13246721525565905No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG14820.13211064132495742No Hit
CTACTGTTGCTCATATACAAGTAGTGGCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCC14790.13184321087693118No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14760.13157578042890494No Hit
GTCCAGGAGATCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC14610.1302386281887738No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC14600.13014948470609838No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACAATCTTTGGGCTCCAGG14430.1286340455006164No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14100.12569231057232788No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13900.12390944091881971No Hit
GTATATGAGCAACAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAAA13880.12373115395346888No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATTTTGGGAATTATAACCTTGTCTCCTGGT13800.1230180060920656No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13740.12248314519601314No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC13730.12239400171333774No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13720.12230485823066232No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13700.12212657126531151No Hit
GGACGAGGCTGATTACTACTGTTGCTCATATACAAGTAGTGGCACTTTGGTCTTCGGCGGAGGGACCAAGCTGAC13550.12078941902518037No Hit
TTACTACTGTTGCTCATATACAAGTAGTGGCACTTTGGTCTTCGGCGGAG13520.12052198857715414No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13500.1203437016118033No Hit
CAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATTGTCAGGGA13500.1203437016118033No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC13500.1203437016118033No Hit
GGCTGAGGACGAGGCTGATTACTACTGTTGCTCATATACAAGTAGTGGCA13430.11971969723307543No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13410.11954141026772462No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT13140.11713453623548856No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13020.11606481444338364No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGT12740.11356879692847215No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12680.11303393603241971No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12560.11196421424031477No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12360.11018134458680659No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGAC12230.10902247931202624No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12130.10813104448527215No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12090.10777447055457053No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT11900.10608074438373774No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11880.1059024574183869No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC11700.10429787473022953No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC11650.10385215731685249No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC11620.10358472686882625No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11480.10233671811137053No Hit
AGGTTATAATTCCCAAAATCACTGCTGGTTCCAGTGCAGGAGATGGTGAT11450.10206928766334428No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11400.10162357024996725No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT11330.10099956587123937No Hit
CAACAACACCCAGGCAAAGCCCCCAAATTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG11270.1004647049751869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)