FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10509
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT468644.59035112760491No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC7206.851270339708821No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG3393.225806451612903No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1961.8650680369207344RNA PCR Primer, Index 39 (95% over 23bp)
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC1171.1133314302026833No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT700.6660957274716909RNA PCR Primer, Index 39 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCCGT650.618517461223713No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG610.5804548482253307No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT550.5233609287277572No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGT470.4472357027309925No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC430.40917308973261013No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA360.3425635169854411No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTA350.33304786373584544No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT340.3235322104862499No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG290.27595394423827196RNA PCR Primer, Index 39 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT280.2664382909886764RNA PCR Primer, Index 39 (95% over 22bp)
GAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT250.23789133123988962No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC230.21886002474069843No Hit
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC210.19982871824150727No Hit
CATATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT190.18079741174231612No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG180.17128175849272054No Hit
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA160.15225045199352938No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCC150.14273479874393377No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG140.1332191454943382RNA PCR Primer, Index 39 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG130.12370349224474261Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACCTCTCGTATGCCGT130.12370349224474261No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT130.12370349224474261No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA130.12370349224474261RNA PCR Primer, Index 39 (96% over 30bp)
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG120.11418783899514702RNA PCR Primer, Index 39 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGCCCTCTCTACATCTCGTATGCCGT120.11418783899514702No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA120.11418783899514702RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG110.10467218574555144RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3750.074.7387470
CAGCTCT201.11250585E-464.761915
AGCTCTT201.11250585E-464.761916
GCTCTTA201.11250585E-464.761917
ACAGCTC201.11250585E-464.761914
ATTGGAG150.002945506564.76193
TCATTGG150.002945506564.76191
TGGGGGG506.017217E-964.32432670
AAAGTGG200.00396007260.00000467
AAAAAGT200.004246735558.9595465
AAGGGGG4200.058.87445469
AAAGGGG4700.051.98114468
AAAAGGG4750.050.52631867
AAAAAGG4850.049.7242966
CTTGAAA6900.049.54760457
TGAAAAA6950.049.4644359
GCTTGAA6900.049.27536456
TTGAAAA6950.049.19114358
GAAAAAA7100.048.95148560
TGCTTGA6950.048.92086455
Produced by FastQC (version 0.11.5)