FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156964
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26030.22498539280392474No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC16220.14019450907720551No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT15980.13812011436829497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6200.045.5671351
GGTATCA19750.042.4029771
TGGTATC6550.041.5774232
GTATCAA52850.039.392161
ATCAACG64000.032.5096133
TCAACGC64100.032.4617044
CAACGCA64800.032.111045
AACGCAG65900.031.6770636
CGGGTAT1400.031.22243317
TATCAAC66600.031.1980972
ACGCAGA69000.030.253897
CGCAGAG69900.029.8175538
GACCGGA3050.029.64730670
GCAGAGT72850.028.6550249
AGAGTAC72900.028.59049411
CAGAGTA74000.028.20970710
GAGTACG58500.025.7464412
AGTACGG59300.025.402413
CGACGTT750.001123183625.38003767
GGCTATA801.1367479E-425.2246651
Produced by FastQC (version 0.11.5)