FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311760
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.2970233512958686No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT6980.22389017192712343No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5690.18251218886322812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5280.1693610469591994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5270.16904028740056454No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5010.1607005388760585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.14402104182704645No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4480.1437002822684116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4260.13664357197844496No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4150.13311521683346164No Hit
GAGTTACACTCACCCCCTCACTTTCGGCGGAGGGACCAACGTGGCGATCAAACGAACTGTGGCTGCACCAT4130.13247369771619194No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC4070.13054914036438287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3910.12541698742622528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3840.12317167051578137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3730.11964331537079806No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC3600.11547344110854503No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3480.11162432640492687No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3210.102963818321786No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG3160.10136002052861175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3130.1003977418527072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG250.001193396581.861670
CCGTAGC200.006729204752.9240643
CGTATGC850.049.4224141
TCGTTAT250.0095474948.43968666
TTCGTTA250.00996892647.91306765
GCCGTCT900.047.64278446
GTAGTTT753.6396203E-545.4786770
TACGTAT606.657143E-445.4786770
ATCTCGT752.0008883E-1145.4479737
TGCTTGA1000.045.0375755
CCGTCTT1000.043.0842947
ACGGGCT407.22284E-540.50888416
GTATGCC1050.040.17572442
TTATACA1050.040.1102142
ACTAGGC905.456968E-1239.9936727
TCGTATG1050.039.76054440
CGTCTTC1100.039.34235848
TATACAC1000.038.8760573
GTATCAA25000.037.332991
ATACACA1050.037.0248154
Produced by FastQC (version 0.11.5)