FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618244
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCC26760.4328388144486643No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCT26080.4218399208079658No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25540.41310550526976403No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23430.3789765852964202No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23110.3738006353478562No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22140.35811103706627156No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22000.3558465589637748No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21710.3511558543228887No Hit
GACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGA19690.3184826702725785No Hit
GTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCAC19400.3137919656316923No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18790.3039253110422422No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17750.2871034737094092No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16290.26348820206908596No Hit
CCATTAATCCCGATATTGGAGGCGCTTCCAGAACAAGAAATGGTGACCCT15130.2447253835055415No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14160.22903578522395687No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13780.22288934466003713No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13710.22175710560878875No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13680.22127186030111087No Hit
GCCTTGGGCTGACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGACTGTCATCCCACG13360.2160959103525469No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAG13300.21512541973719113No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12930.209140727609164No Hit
GGATTAATGGTGTGAACTGGTACCAGCAACTCCCGGGAGCGGCCCCCAGA12900.20865548230148614No Hit
ATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12740.20606750732720414No Hit
GTCTGAGAGGGCATGTCTTCGCAACTGGGACCAAGGTCACCATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA12640.2044500229682779No Hit
GTCTGAGGATGAGGCTGATTATTTTTGTGCAGCGTGGGATGACAGTCTGAGAGGGCATGTCTTCGCAACTGGGAC12310.19911232458382128No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGATGGTGACCTTGGTC12020.19442161994293514No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11440.1850402106611629No Hit
TCCTCAGACTGGAGGCCACTGATGACCAGGGAGGCTGAGGTGCCAGACCTGGAGGCAGAGAATCGGTCAGGGACC11230.1816434935074178No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCA10970.17743803417420956No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10960.1772762857383169No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGCCCAGT10950.17711453730242427No Hit
AGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAAGCG10830.17517355607171278No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10670.17258558109743077No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10540.17048285143082667No Hit
CCCCTGAGGGCCGCTCATTATTATCAGAGATGAGGAGTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCA10500.16983585768725618No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10450.16902711550779304No Hit
GTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCACACCATTAATC10060.16271892650798067No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC9560.15463150471334944No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGCCTCCAGT9490.15349926566210104No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGC9390.1518817813031748No Hit
ACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGAC9290.15026429694424856No Hit
GGTCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTTTTGTGCAG9180.14848506414942966No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGT8970.14508834699568457No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8910.1441178563803288No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8840.1429856173290804No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT8760.14169162984193942No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8740.1413681329701542No Hit
GGATGACAGTCTGAGAGGGCATGTCTTCGCAACTGGGACCAAGGTCACCATCCTAGGTCAGCCCAAGGCCAACCC8520.13780966738051642No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8520.13780966738051642No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8420.13619218302159017No Hit
GCCCCCAGACTCCTCATCTCTGATAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGT8410.13603043458569755No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8290.13408945335498607No Hit
CCTGAGGGCCGCTCATTATTATCAGAGATGAGGAGTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCACA8210.13279546586784508No Hit
CCTCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCAC8050.13020749089356307No Hit
CCTCATCTCTGATAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAGC8050.13020749089356307No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7880.12745776748338844No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCG7870.12729601904749582No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC7820.12648727686803268No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7800.12616377999624745No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCAC7730.12503154094499905No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT7720.12486979250910643No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC7640.12357580502196544No Hit
CTCTCAGACTGTCATCCCACGCTGCACAAAAATAATCAGCCTCATCCTCA7470.12082608161179081No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7440.12034083630411295No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7430.1201790878682203No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7380.1193703456887572No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7220.1167823707144752No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG7100.1148413894837637No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7070.1143561441760858No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7020.11354740199662268No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC6990.11306215668894481No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6960.11257691138126694No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6780.1096654395351997No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT6660.1077244583044882No Hit
TCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAG6650.10756270986859556No Hit
CTGATGACCAGGGAGGCTGAGGTGCCAGACCTGGAGGCAGAGAATCGGTC6540.1057834770737767No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6240.10093102399699795No Hit
GTCATCCCACGCTGCACAAAAATAATCAGCCTCATCCTCAGACTGGAGGC6220.1006075271252127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)