FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618244
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCC24300.3930486992190786No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24270.3925634539114007No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCT24180.391107717988367No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22800.36878643383518483No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21610.3495383699639624No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20640.33384877168237787No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20030.32398211709292773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19520.31573294686240383No Hit
GACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGA19390.3136302171957997No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18970.30683678288830946No Hit
GTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCAC18430.2981023673501077No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18110.29292641740154374No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17120.2769133222481739No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15860.2565330193257031No Hit
GCCTTGGGCTGACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGACTGTCATCCCACG14360.23227075394180938No Hit
CCATTAATCCCGATATTGGAGGCGCTTCCAGAACAAGAAATGGTGACCCT14280.23097676645466836No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14280.23097676645466836No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14150.22887403678806426No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13470.21787514314736575No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGATGGTGACCTTGGTC12960.20962597291684187No Hit
GTCTGAGAGGGCATGTCTTCGCAACTGGGACCAAGGTCACCATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA12530.20267079017345901No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12530.20267079017345901No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12500.20218554486578114No Hit
GTCTGAGGATGAGGCTGATTATTTTTGTGCAGCGTGGGATGACAGTCTGAGAGGGCATGTCTTCGCAACTGGGAC12280.19862707927614337No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAG12050.19490686525061302No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11670.1887604246866933No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11450.18520195909705553No Hit
GGATTAATGGTGTGAACTGGTACCAGCAACTCCCGGGAGCGGCCCCCAGA11390.1842314684816998No Hit
ATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11050.17873202166135055No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCA10900.17630579512296116No Hit
GTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCACACCATTAATC10610.17161509048207502No Hit
TCCTCAGACTGGAGGCCACTGATGACCAGGGAGGCTGAGGTGCCAGACCTGGAGGCAGAGAATCGGTCAGGGACC10540.17048285143082667No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGCCCAGT10480.1695123608154709No Hit
AGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAAGCG10160.16433641086690692No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10120.1636894171233364No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9780.15818997030298715No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC9630.1557637437645978No Hit
CCCCTGAGGGCCGCTCATTATTATCAGAGATGAGGAGTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCA9630.1557637437645978No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9500.15366101409799368No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9230.1492938063288928No Hit
ACCTAGGATGGTGACCTTGGTCCCAGTTGCGAAGACATGCCCTCTCAGAC9050.14638233448282556No Hit
CTCTCAGACTGTCATCCCACGCTGCACAAAAATAATCAGCCTCATCCTCA8790.14217687514961733No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8690.14055939079069105No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8620.1394271517394427No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8620.1394271517394427No Hit
GGATGACAGTCTGAGAGGGCATGTCTTCGCAACTGGGACCAAGGTCACCATCCTAGGTCAGCCCAAGGCCAACCC8610.13926540330355006No Hit
GGTCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTTTTGTGCAG8550.1382949126881943No Hit
GTCATCCCACGCTGCACAAAAATAATCAGCCTCATCCTCAGACTGGAGGC8300.13425120179087868No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGT8290.13408945335498607No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGC8280.13392770491909345No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8240.13328071117552293No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGCCTCCAGT8230.1331189627396303No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG7990.1292370002782073No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCG7990.1292370002782073No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7760.12551678625267693No Hit
CCTCATCTCTGATAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGTCTGGCACCTCAGC7730.12503154094499905No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7710.12470804407321381No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7690.12438454720142857No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT7640.12357580502196544No Hit
CCTGAGGGCCGCTCATTATTATCAGAGATGAGGAGTCTGGGGGCCGCTCCCGGGAGTTGCTGGTACCAGTTCACA7630.12341405658607282No Hit
GCCCCCAGACTCCTCATCTCTGATAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAGGT7560.12228181753482445No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC7560.12228181753482445No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCACCCTCAGCGGCTGGGACCCCCGGGCAGAGGGTCAC7260.11742936445804568No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7260.11742936445804568No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC7190.1162971254067973No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7010.11338565356073006No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6890.11144467233001856No Hit
CCTCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGAGTCAGCCAC6860.1109594270223407No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6850.11079767858644808No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6770.10950369109930706No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC6760.10934194266341445No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6670.10788620674038081No Hit
GGGCATGTCTTCGCAACTGGGACCAAGGTCACCATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC6430.10400424427895781No Hit
CTGATGACCAGGGAGGCTGAGGTGCCAGACCTGGAGGCAGAGAATCGGTC6350.10271025679181682No Hit
CTGTCATCCCACGCTGCACAAAAATAATCAGCCTCATCCTCAGACTGGAG6190.10012228181753483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)