FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932801
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16700.1790306828573297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15290.1639149186160821No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA14710.1576970865168455No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT14280.15308731444327353No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14180.15201527442616378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13680.14665507434061498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13550.1452614223183723No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13190.14140207825677717No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12720.13636349017636132No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12660.13572026616609545No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12360.13250414611476616No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12350.1323969421130552No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12290.13175371810278932No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGAATTAC12260.1314321060976564No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11650.1248926619932869No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11600.12435664198473201No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11460.12285578596077835No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11350.12167654194195762No Hit
GTCTATTACTGTGCGATCTTTCTTTGGAATGGTTATTATCCGGAGGCTGA11310.12124772593511371No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11210.12017568591800394No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGCCAGCTTGGTCCCTC10590.11352903781192344No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10340.11084893776914906No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG10230.10966969375032833No Hit
GCCTTGGGCTGACGTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCCAGTGATCAGTAAGAACATCCCACAC9920.10634636969728806No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9750.10452390166820145No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9520.10205820962884904No Hit
CCCCAGGACAGACGGCCAGAATTACCTGTGGGGGAAACGACATTGGAAGT9360.10034294560147342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)