FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932801
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24950.26747398426888475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16930.18149637489668213No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14260.1528729064398516No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14240.15265849843642965No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13490.14461819830810643No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA13350.1431173422841528No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12970.1390435902191357No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12720.13636349017636132No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12440.13336177812845398No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12370.13261135011647715No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGAATTAC12320.13207533010792227No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12200.13078888208739056No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11650.1248926619932869No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11640.12478545799157592No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11120.11921084990260516No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11080.11878203389576127No Hit
GTCTATTACTGTGCGATCTTTCTTTGGAATGGTTATTATCCGGAGGCTGA10610.11374344581534539No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10370.11117054977428198No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG9930.10645357369899905No Hit
GCCTTGGGCTGACGTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCCAGTGATCAGTAAGAACATCCCACAC9630.10323745364766977No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9540.10227261763227097No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGCCAGCTTGGTCCCTC9540.10227261763227097No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9530.10216541363055999No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9420.10098616961173926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTT150.002673555966.8298529
GCTATCG200.00846805849.94376427
CGCGCTT200.00869436349.61158812
CAAGCTA606.64544E-445.51513370
GGTATCA16950.041.382921
GTATCAA45450.038.7286831
GTGGTAT5050.037.3455161
TCTGGAG5700.033.53746470
TGGTATC5650.033.374252
GCGTCGT3850.033.37404662
TATCAAC52550.033.2388732
TAAGGGT400.00319349333.072624
TCGTCTC3850.032.97241265
ATCAACG54550.032.011633
TCAACGC54550.031.8903754
TTGGCGT3900.031.44906859
CAACGCA55350.031.3696965
GTCGTCT4150.031.31652664
AACGCAG55600.031.2252966
TACGTAT1103.462496E-431.03304370
Produced by FastQC (version 0.11.5)