FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734136
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTGTTCA60630.8258687763575141No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT52610.7166247125873135No Hit
GTGTTCACTCTCACCATCAGCAGTCTGCACCCTGAAGATTTTGCAACTTATTATTGTCAACAGAGTTTCAGTACC47530.6474277245632961No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40320.5492170388047991No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28570.38916495036342036No Hit
GAGTGAACACTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA28390.3867130885830418No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26460.3604236817156494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25690.34993516187736334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24060.32773219131060183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22790.3104329443045975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20650.2812830320267634No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20210.27528959211917137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19780.26943236675493365No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTATCAAGTATTTGAATTGGTATCAGCAAAAA19770.2692961522115793No Hit
CCTTGGTACCTCCGCCGAAACTGAGCTCGGGGGTACTGAAACTCTGTTGA19120.26044220689354564No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18840.25662819967962336No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT18430.2510434034020944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17940.24436889077773055No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17710.24123595628058017No Hit
GTACCAAGGTGGAGGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC17380.23674087634988614No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTC17200.23428901456950757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17000.23156472370242023No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16790.2287042182919786No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16690.22734207285843494No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16640.22666100014166313No Hit
ATCTGGGACAGTGTTCACTCTCACCATCAGCAGTCTGCACCCTGAAGATT16560.2255712837948282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16530.2251626401647651No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16470.2243453529046389No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16400.2233918511011584No Hit
GTGCAGCCACAGTTCGCTTGACCTCCACCTTGGTACCTCCGCCGAAACTG15720.21412926215306155No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15440.21031525493913936No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15030.2047304586616104No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT14980.20404938594483857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14360.19560408425686793No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGACCTCCACCTTGGTAC13980.19042793160940208No Hit
GTGCCAGATGTGTCATCCAGATGACCCAGTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13660.1860690662220624No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13200.1798031972277616No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG13160.17925833905434416No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12650.17231139734327153No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTCTCCAGCCTCC12470.16985953556289296No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12450.16958710647618425No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12400.16890603375941243No Hit
GTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11560.15746401211764577No Hit
GGGCATTATCAAGTATTTGAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATACTGCGTC11520.1569191539442283No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11510.15678293940087396No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11430.15569322305403904No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11210.15269650310024302No Hit
ATCATACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAACAACACACTGTATCTA11070.15078949949328188No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10880.14820142316954896No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10750.14643063410594223No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10570.14397877232556364No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10530.1434339141521462No Hit
GTCTGCACCCTGAAGATTTTGCAACTTATTATTGTCAACAGAGTTTCAGT10310.14043719419835018No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10210.13907504876480653No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10140.13812154696132595No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTGTTCACT10070.13716804515784542No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTGTTCACT10040.13675940152778232No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9710.13226432159708829No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9610.13090217616354463No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9550.13008488890341843No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9400.12804167075310297No Hit
GTGTCATCCAGATGACCCAGTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGG9360.1274968125796855No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9180.12504495079930694No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9090.12381901990911766No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9080.1236828053657633No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9080.1236828053657633No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9050.1232741617357002No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9020.1228655181056371No Hit
CAGTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTCAAATACTTGATAATGCCCTGA8930.1216395872154478No Hit
ATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTGTTCACTCTCA8900.12123094358538473No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8870.12082229995532164No Hit
GATCTGGGACAGTGTTCACTCTCACCATCAGCAGTCTGCACCCTGAAGAT8770.11946015452177798No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8770.11946015452177798No Hit
ATATTATTGTGCGAAAGATGTAAGAAGACTTTTGACTGGTTCTTCCTCCT8710.11864286726165178No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8690.11837043817494307No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8670.11809800908823434No Hit
GTGTATGATGGCACCACTACCACTAATAATTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGATCCAGGT8670.11809800908823434No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8530.11619100548127322No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8390.11428400187431212No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8270.11264942735405974No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8270.11264942735405974No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8210.11183214009393355No Hit
GCCCTATATTATTGTGCGAAAGATGTAAGAAGACTTTTGACTGGTTCTTCCTCCTCAGGTTTTGACTACTGGGGC8210.11183214009393355No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8080.1100613510303268No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8030.10938027831355497No Hit
GCCACAGTTCGCTTGACCTCCACCTTGGTACCTCCGCCGAAACTGAGCTC8010.10910784922684624No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8000.10897163468349187No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7990.10883542014013753No Hit
GAGTTTCAGTACCCCCGAGCTCAGTTTCGGCGGAGGTACCAAGGTGGAGGTCAAGCGAACTGTGGCTGCACCAT7990.10883542014013753No Hit
GTCTCCTACAGATGCAGACAGGGAGGCTGGAGACTGGGTCATCTGGATGA7970.1085629910534288No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7780.10597491472969585No Hit
GGGTGCAGACTGCTGATGGTGAGAGTGAACACTGTCCCAGATCCACTGCC7720.10515762746956968No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7710.10502141292621531No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7690.10474898383950658No Hit
GGTGGAGGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC7550.10284198023254545No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7470.10175226388571056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG7450.10147983479900183No Hit
CTCTCACCATCAGCAGTCTGCACCCTGAAGATTTTGCAACTTATTATTGTCAACAGAGTTTCAGTACCCCCGAG7410.10093497662558436No Hit
GTTCAGTGGCAGTGGATCTGGGACAGTGTTCACTCTCACCATCAGCAGTC7390.10066254753887563No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7360.10025390390881253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)