FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948640
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34560.364311013661663No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG34180.360305279136448No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTACCAGTATCCCACAC31910.33637628605161074No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29650.3125527070332265No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA29010.30580620678023274No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA25700.27091415078428066No Hit
GGATTACCTGTGGGGGAGACAACATTGGATATAAAATTGTTCACTGGTACCAGCAGACGCCAGGCCAGGCCC25440.26817338505650196No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24200.25510204081632654No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21660.22832686793725754No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20790.21915584415584413No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20610.21725839095968966No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAGACAACATTGGATATAAAATTGTTCACTGGTACCAGCAGA20330.2143067970990049No Hit
ATATATTACTGTGCGAGAAGTAAAAAGGGTGGGAGCTCCTACCCCTGGTACTTCGATCTCTGGGGCCGTGGCACC20310.21409596896609884No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19210.2025004216562658No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19180.20218417945690673No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT18680.19691347613425536No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18630.19638640580199024No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18620.1962809917355372No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17960.18932366334963738No Hit
CCCCTGTGGTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT17390.1833150615618148No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTATCATCATAGA17260.18194467869792544No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC16850.1776227019733513No Hit
CCACAGGGGCCTGGCCTGGCGTCTGCTGGTACCAGTGAACAATTTTATAT16810.17720104570753922No Hit
GTATCATCATAGACGACCACCACAGGGGCCTGGCCTGGCGTCTGCTGGTA16670.17572524877719684No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC16440.1733007252487772No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15780.16634339686287739No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15750.1660271546635183No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC15680.1652892561983471No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15420.1625484904705684No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15350.16181059200539719No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14840.15643447461629278No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14670.15464243548659135No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14440.1522179119581717No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14440.1522179119581717No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14400.15179625569235958No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14360.1513745994265475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14360.1513745994265475No Hit
CTATTACTGTCAGGTGTGGGATACTGGTAGTGATCATGTGGTATTCGGCG14320.15095294316073538No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14310.15084752909428234No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG14280.15053128689492326No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14280.15053128689492326No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14210.14979338842975207No Hit
CTCCTGTGCAGCCTCTGGGTTCACCATCAGTTACAACTACATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGC13850.1459984820374431No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13630.14367937257547647No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA13580.14315230224321132No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATACCGACCGGCCCTCAGG13560.14294147411030528No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13260.13977905211671446No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13170.1388303255186372No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13090.137987012987013No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12800.13493000505987518No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT12770.1346137628605161No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12760.13450834879406307No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12550.1322946533985495No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12490.13166216899983132No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12480.1315567549333783No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC12450.1312405127340192No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTAC12390.13060802833530105No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC12380.13050261426884804No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12030.12681312194299207No Hit
CTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC11730.12365069994940125No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11610.12238573115196491No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11580.12206948895260583No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11580.12206948895260583No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11570.12196407488615281No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC11530.1215424186203407No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11400.12017203575645134No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11120.11722044189576657No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10990.11585005903187721No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG10830.11416343396862877No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10820.11405801990217573No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10740.11321470737055152No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10700.11279305110473942No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10640.11216056670602126No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGA10530.11100101197503795No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10360.10920897284533648No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10300.10857648844661831No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCA10240.10794400404790015No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG10210.10762776184854107No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10060.10604655085174566No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10020.10562489458593353No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10000.10541406645302748No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT9960.10499241018721538No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9940.10478158205430932No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9900.10435992578849722No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGATGTCTCTGCAAATGAA9840.10372744138977905No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAGACAACATTGGATATAAAATTGTT9780.10309495699106089No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9680.10204081632653061No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9560.10077584752909427No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9530.10045960532973519No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9520.10035419126328217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT150.002110405670.9375860
GTATCAA18050.040.1354981
TGGTATC2000.039.2728962
TATCAAC19250.037.432332
TCAACGC20100.035.8531534
ATCAACG20050.035.7684443
CAACGCA20600.034.982935
AACGCAG20550.034.9036876
CTCTGGA10150.034.6922769
AATGGTA4150.033.7433970
ACGCAGA21300.033.5143287
CGCAGAG21300.033.5143288
CAGAGTA21400.033.03502310
AGAGTAC21500.032.88310611
GCAGAGT21550.032.808549
TCTGGAG16400.031.30865370
CCCTATA553.4570266E-431.0457732
ATTGTCT10450.030.1457964
TCGTGTT10200.029.83937551
GAATTGT10700.029.31991262
Produced by FastQC (version 0.11.5)