FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271072
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC76040.5982351904534126No Hit
CCCCTATACTTGCCATCTTTGGTAACGACAACCGGCCCTCAGGGATCCCA69160.5441076508647819No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC59810.4705476951738375No Hit
GTATAGGGGCCTGTCCTGGCTTCTGTTGGTACCAGCTTGCATCAAACTTT56260.4426185141360993No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC48710.3832198333375293No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40870.321539613806299No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37580.2956559502530148No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34790.2737059741698346No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32950.25923000427985193No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATCTTCACCA32860.25852194053523325No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC32760.25773520304121245No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32620.2566337705495833No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30090.23672931195085722No Hit
AGCCAGGACAGGCCCCTATACTTGCCATCTTTGGTAACGACAACCGGCCC29530.23232358198434078No Hit
CTATTACTGTAACTCCCGGGACACCAGTGGTGAAGATGTGGTTTTCGGCG28360.22311875330429748No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27580.21698220085093525No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27460.21603811585811034No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26540.20880013091311903No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25990.20447307469600462No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC25650.20179816721633392No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA25180.19810050099443618No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT25160.19794315349563205No Hit
GACTATTACTGTAACTCCCGGGACACCAGTGGTGAAGATGTGGTTTTCGG24760.19479620351954885No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23990.18873832481558875No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23950.18842362981798041No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22990.18087094987538077No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22740.17890410614032878No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22710.17866808489212255No Hit
GTCCTGGCTTCTGTTGGTACCAGCTTGCATCAAACTTTCTGAGACTGTCTCCTTGGCATGTGATCCTGACTGT22710.17866808489212255No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG22670.17835338989451424No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA22620.17796002114750384No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22340.17575715616424561No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22310.17552113491603938No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA20860.16411344125273783No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20700.16285466126230455No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19820.15593137131492157No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTCTCAGAAAGTTTGATGCAAGCTGGTA19140.15058155635558015No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18670.14688389013368242No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18420.14491704639863046No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTTCACTTTCAGGGAGAACTGGCTCTTGGACGTGTCCACTGA18290.1438942876564034No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18090.14232081266836183No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17870.14058999018151608No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17840.14035396893330984No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17430.13712834520782458No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16710.13146383525087485No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16360.12871025402180208No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16060.1263500415397397No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAT16010.1259566727927293No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG16000.12587799904332722No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15450.12155094282621283No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15290.12029216283577958No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG14790.11635847536567558No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14780.1162798016162735No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG14130.11116600790513835No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14100.11092998665693211No Hit
ACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTCTCAGAAAGTTTGATG14080.11077263915812793No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13950.1097498804159009No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13870.10912049042068428No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13780.10841242667606556No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA13720.10794038417965307No Hit
GTCAGGATCACATGCCAAGGAGACAGTCTCAGAAAGTTTGATGCAAGCTGGTACCAACAGAAGCCAGGACAGGCC13680.10762568918204476No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13480.10605221419400317No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13440.10573751919639486No Hit
GCTTGGTCCCTCCGCCGAAAACCACATCTTCACCACTGGTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT13360.10510812920117821No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13340.10495078170237407No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATCTTCAC13250.10424271795775535No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGGT13150.10345598046373455No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13130.10329863296493037No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13010.10235454797210544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.10023035673824929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA1053.3542165E-948.74930270
GGTATCA12450.046.1136781
GTATCAA36700.041.250331
TGGTATC2950.040.5321042
GTGGTAT3100.039.6853871
TACACGG451.0612631E-437.9586375
TCGTATG1650.037.66859440
ATCTCGT1950.037.07068637
CGTATGC1500.036.87843741
TATCAAC41000.036.8291322
ATCAACG42000.035.870913
TCAACGC42400.035.5325054
TCTCGTA1950.035.34745438
TATCTCG2050.035.22466336
ATGTGAT25100.035.17815470
CAACGCA43000.035.1161545
AACGCAG44000.034.3180586
CTTAGTC400.002724550434.1627732
ACCGATC2400.032.77026427
TTGGACG5250.032.44587759
Produced by FastQC (version 0.11.5)