FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631652
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19660.3112473323918867No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCATTCTCAGGGAGA15820.2504543641118844No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13460.213092019023133No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13290.2104006636565704No Hit
GCCTTGGGCTGACCCAGGACGGTCAATTTGGTCCCTCCGCCGAAAAGCCCCAAATTGCTGCTGCCTTCATATGAG12110.1917194911121947No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11230.17778776921469416No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11150.17652124904219413No Hit
GTCTATTACTGTGCGAGAGATATAGTGGGAAGCACTTTCTGGTTCGACCC10630.1682888679209438No Hit
GTGTGGTGGTCTCCACTCCCGCCCTGACGGGGCTGCTATCTGCCTTCCAG10070.15942322671344347No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9580.15166579065688068No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9520.15071590052750566No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9410.1489744352903181No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9130.14454161468656793No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9050.1432750945140679No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9010.14264183442781786No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8570.1356759734790676No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8490.13440945330656756No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8450.1337761932203175No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8380.13266798806937996No Hit
GTATCTGGTGGGTCCGTCACCACTACAAACTGGTGGAGTTGGGTCCGCCG8150.12902674257344232No Hit
GATTATTACTGCAGCTCATATGAAGGCAGCAGCAATTTGGGGCTTTTCGG8100.1282351674656298No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7790.12332740179719212No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAATTTGGTCCCTC7670.12142762153844205No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7650.12111099149531704No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7200.11398681552500427No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7140.11303692539562923No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7120.11272029535250422No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7100.1124036653093792No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6960.11018725500750413No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6890.10907904985656659No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6850.10844578977031658No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6750.10686263955469151No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG6740.10670432453312899No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6730.1065460095115665No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCCTGA6710.10622937946844148No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6670.10559611938219148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.10306307903719136No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6500.10290476401562886No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGTGACGTT6470.10242981895094135No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6410.1014799288215663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1250.046.6562238
ACGAGAC1300.046.2932322
ATGCCGT1400.044.96267344
CGTATGC1400.044.5933141
AATTGAA4950.044.1436770
AGGGGGG1900.043.127270
ATCTCCG1400.042.9695510
TCGTATG1450.042.92513740
GAAACGT350.001076048141.27272455
CCGTCTT1550.040.93696247
ATCTCGT1450.040.10032337
GTATGCC1600.039.13810742
CGAGGCT1550.039.0678929
AGACCGA1600.037.64603825
CTTATAC1650.036.4763371
CGGATGA1151.074688E-535.62681670
CGTCTTC1850.034.393348
CACGAGA1750.034.3838121
GATCTCG1700.034.1092736
GAGACCG1800.033.4498924
Produced by FastQC (version 0.11.5)