FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631652
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17660.2795843280793855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14340.22702374092063352No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCATTCTCAGGGAGA13910.22021619499344577No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12920.20454300785875765No Hit
GCCTTGGGCTGACCCAGGACGGTCAATTTGGTCCCTCCGCCGAAAAGCCCCAAATTGCTGCTGCCTTCATATGAG11930.18886982072406958No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11450.18127069968906928No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11330.17937091943031924No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10510.16638908766219374No Hit
GTGTGGTGGTCTCCACTCCCGCCCTGACGGGGCTGCTATCTGCCTTCCAG10450.1654391975328187No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10090.1597398567565685No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10010.15847333658406845No Hit
GTCTATTACTGTGCGAGAGATATAGTGGGAAGCACTTTCTGGTTCGACCC9850.15594029623906835No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9600.1519824207000057No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9450.1496076953765681No Hit
GTATCTGGTGGGTCCGTCACCACTACAAACTGGTGGAGTTGGGTCCGCCG8630.13662586360844262No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8430.1334595631771925No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8090.1280768524440673No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8000.12665201725000477No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7750.1226941417109421No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7700.12190256660312958No Hit
GATTATTACTGCAGCTCATATGAAGGCAGCAGCAATTTGGGGCTTTTCGG7660.12126930651687955No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7650.12111099149531704No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7560.11968615630125448No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7450.11794469106406692No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG7390.11699480093469189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7310.11572828076219185No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7210.11414513054656679No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7180.11367018548187928No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC7170.11351187046031676No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7050.11161209020156668No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCCTGA6950.11002893998594164No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6800.10765421466250405No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6770.10717926959781651No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAATTTGGTCCCTC6770.10717926959781651No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6690.10591274942531646No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGTGACGTT6520.10322139405875387No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCCTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6440.10195487388625384No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6350.10053003869219126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT350.001453947738.82240333
AATTGAA5800.037.85560670
ATGCGCT400.00284923933.84966331
TAGCTCG400.003039001733.4073410
GTATCAA28050.032.902661
TGCGTTA553.3868666E-431.1523937
CGCAATT603.7824456E-430.57037560
TATCAAC31050.029.4989192
CGCAGAG31000.029.2044838
CATGCCG950.00618191528.88980570
CGAAATT500.00639298428.69622653
AGAGTAC31350.028.66304211
ATCAACG31950.028.6475323
CGGGTAC704.787695E-528.639417
GGTATCA13550.028.626851
GGGGCGA500.00657559428.53124851
CAGAGTA31500.028.5288110
ATGCGTT605.7269086E-428.4800136
GCAGAGT31450.028.467949
ACGGGAT2000.028.40073816
Produced by FastQC (version 0.11.5)